Holt was down to his last 17 players for last week’s 30-18 defeat by Whitehaven.
However, Holt was encouraged by his side’s performance last week.
He said: “One of the key areas for us to keep up the effort and commitment that we saw at Whitehaven. The lads proved there that they will put their bodies on the line.”
Sheffield Eagles coach Mark Aston is aiming to maintain his side’s momentum after they moved to top spot in the Kingstone Press Championship table with a 38-22 win over Hunslet Hawks on Wednesday night.
A second game in four days will test Aston’s squad, but Michael Knowles is set to return after being rested for the Hawks clash.