Wigan will be playing one of their Super 8s games at The Den, in Southwark, the home of soccer club Millwall FC.
The Warriors threrefore become the first club to take a Super League home game on the road to London.
The game against Catalans Dragons on Saturday September 5 will take place at The Den.
Wigan chairman Ian Lenagan acknowledged that some supporters may be disappointed at the move, but it was part of a long-term vision for success that the club has.
“We are taking a Super League game to London and we intend to leave our mark” said Lenagan.
“This is no half-hearted measure. It has been well planned and is underpinned by a strong community programme, the development of our London Business Club and the opening of Wigan’s office on Buckingham Palace Road.
“The fixture provides an opportunity for Wigan and rugby league fans to sample live Super League rugby in the capital.
“We understand that some of our fans in and around Wigan may feel a little disgruntled by the move; however, we sincerely hope that they can understand the opportunity this provides us to grow as a club.
“With a bigger fan base, more blue chip corporate support and access to a wider talent pool of players it will allow us to meet our mission to become the biggest club in the world.”