League 1 Cup holders Keighley will face Doncaster in the first round of the competiton.
Hunslet will take on Newcastle, while Cumbrian rivals Whitehaven and Workington Town will meet.
The first round ties will be played over the weekend of February 18-19.
The draw in full is as follows:
Pool A (northern regions):
Whitehaven v Workington Town
Hunslet RLFC v Newcastle Thunder
Keighley Cougars v Doncaster
Pool B (midlands and southern regions):
Oxford v Coventry Bears
Hemel Stags v University of Gloucestershire All Golds